
Cryopreservation is a process where cells or whole tissues are preserved by cooling to low sub-zero temperatures, such as 77 K or −196 °C (the boiling point of liquid nitrogen. At these low temperatures, any biological activity, including the biochemical reactions are effectively stopped. This leads to preservation of the sperrms which can be retrieved later using special process.
Human Oocyte cryopreservation is a new technology in which a woman’s eggs (oocytes) are extracted, frozen and stored. Later, when she is ready to become pregnant, the eggs can be thawed, fertilized, and transferred to the uterus as embryos.
Cryopreservation for embryos are used for embryo storage, e.g. when in vitro fertilization has resulted in more embryos than is currently needed then extra embryos may be utilized at a later date. This is one of the most successful cryopreservation techniques.
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